Czech Society for Structural Biology
Česká společnost pro strukturní biologii



The Czech Society
for Structural Biology
(Česká společnost
pro strukturní biologii

Videnska 1083
CZ-142 20 Prague
Czech Republic

CSSB@structbio.org
VAT number: 0022869433
account num.: 2300076583/2010
IBAN: CZ2820100000002300076583
SWIFT/BIC: FIOBCZPPXXX

Proteins for Life - The 12th P4EU meeting

11-12 December 2017,
BIOCEV – Biotechnology and Biomedicine Centre of the Academy of Sciences and Charles University, Průmyslová 595, Vestec
 
Please accept our warm invitation to a conference/workshop "Proteins for Life" which is a title of the 12th meeting of P4EU - an international pan-European informal network of specialists in protein expression, purification and characterization, structural biologists, leaders of core facilities and research centres (www.p4eu.org). The program will consist of series of lectures highlighting the most recent advances within these fields, case studies and research developments, both from our international guests and from national community, with many possibilities for open informal discussions. Student talks are also welcome.
 

Program: Monday, 11th December – Conference Hall (U2.012)

Time

Title of the talk

Speaker

09:30 – 10:00

Gathering and COFFEE

Outside conference hall

10:00 – 10:20

Program Overview & Welcome Address

Prof. Pavel Martásek, BIOCEV Director

10:20 – 10:40

P4EU Introduction

Dr. Sabine Supmann, Max-Planck Institute of Biochemistry

10:40 – 11:10

Combining cell-free protein production with nanoparticle technologies

Dr. Frank Bernhard, Goethe University Frankfurt

11:10 – 11:30

Adaptation of GoldenBac cloning system for FlashBac users

Dr. Peggy Stolt-Bergner, ProTech, Vienna Biocenter Core Facilities

11:30 – 12:00

Expression of IL-7 mutants modulating T-cell signaling in E. coli and P. pastoris

Dr. Ralf Paul, Medivir AB

12:00 – 12:30

The evolution of HEK293 expressions within the VIB Protein Core

Eng. Jurgen Haustraete, Protein
Core, UGent – VIB

12:30– 13:30

LUNCH

BIOCEV Canteen

13:30 – 14:00

Rational design of CHO cell lines

Dr. Stefan Kol, Technical University of Denmark

14:00 – 14:40

KEYNOTE: Stronger, tighter, faster: improving protein stability, activity, and affinity through computational protein design

Dr. Sarel Fleishman, Weizmann Institute of Science

14:40 – 15:10

High-throughput expression of venom toxins in E. coli for drug discovery

Dr. Renaud Vincentelli, AFMB Marseille

15:10 – 15:30

Bovine antibody production and structural insight into the role of the light chain

Dr. Joanne Nettleship, Oxford
Protein Production Facility

15:30 – 16:00

COFFEE BREAK

Outside conference hall

16:00 – 16:30

HTP protein production for drug
discoveries, even small academic labs can hope to make money one day

Dr. Renaud Vincentelli, AFMB
CNRS University Aix Marseille

16:30 – 16:50

Optimization of expression of
extracellular 3’–nuclease/nucleotidase
from Legionella pneumophila in E. coli

Dr. Tomáš Kovaľ, IBT CAS,
BIOCEV

16:50 – 17:10

How to prepare your protein for crystallization trials

Dr. Pavlína Řezáčová, IOCB CAS

17:10 – 17:30

Adaptation of GoldenBac cloning system
for FlashBac users

Dr. Peggy Stolt-Bergner, ProTech,
Vienna Biocenter Core Facilities

17:30 - 18:00

Transient Gene Expression in Hi5 Insect
cells
Joop van den Heuvel, Helmholtz
Centre for Infection Research

18:00 – 19:00

Transfer to DINNER (P4EU/speakers)

Prague City Center


 

Program: Tuesday, 12th December – Seminar Hall (U2.020A / U2.020B)

Time

Title of the talk

Speaker

09:30 – 10:00

Gathering and COFFEE

Outside conference hall

10:00 – 10:10

BCDD PPT – Making Quality Proteins for Translational Research

Dr. Sharon Shepherd, University of Dundee

10:10 – 10:30

Thermofluor Assay: How to make your protein happy

Dr. Jana Škerlová, IOCB CAS

10:30 – 11:00

Guidelines to improve the quality of
purified proteins: focused on antibodies

Dr. Hüseyin Besir, Head of R&D,
Progen Biotechnik, Heidelberg

11:00 – 11:30

Structural mass spectrometry techniques for protein complexes

Dr. Petr Novák / Man, CMS BIOCEV

11:30 – 12:00

New structurally robust protein scaffolds for directed evolution

Dr. Bohdan Schneider, Institute of Biotechnology, CAS, BIOCEV

12:00– 13:00

LUNCH

BIOCEV Canteen

13:00 – 15:00

Tour around core facilities

 

P4EU general discussion

P4EU members

15:00 – 15:30

COFFEE BREAK

Outside conference hall

15:30 – 17:30

Protein Characterization and Interaction Techniques Workshop

Centre of Molecular Structure, BIOCEV – room no. I1.022

17:30 – 18:00

End of meeting, departure